Protein
Kinases Involved in the Meiotic Maturation and Fertilization of Oocyte
FAN Heng-Yu,TONG Chao,CHEN Da-Yuan,SUN
Qing-Yuan*
( State Key Laboratory of Reproductive Biology Institute of Zoology, the
Chinese Academy of Sciences, Beijing 100080, China )
Abstract The meiosis
and fertilization of vertebrate oocyte are extensively regulated by various
protein kinases.Recently, a great progress has been achieved in the studies on
the molecular mechanisms of oocyte maturation, activation and fertilization.
MPF and MAPK were found to be the key modulators of the cell cycle in oocyte,
whose activation and inactivation result in the entry, arrest and exit of
meiosis. Many protein kinases influence the meiosis by stimulating or inhibiting
the activity of MPF and MAPK. Polo-like kinase activates MPF, whereasMos
initiates oocyte maturation and sustains MII arrest by activating MAPK. CaMK II
down-regulates the MPF level through an ubiquitin-dependent pathway, which
leads to the breakthrough of M phase arrest. Furthermore,
p90rsk is involved in themeiosis regulation as a downstream
regulator of MAPK; protein kinase C inducescortical granule exocytosis after
fertilization and inhibits MAPK activity during maturation; and tyrosine
protein kinase family members modulate the calcium release induced by
fertilization. The cooperation of these protein kinases is essential to the
development and fertilization of the oocyte.
Key words oocyte; meiosis;
protein kinase;
fertilization
*Corresponding author: Tel,
86-10-62563923; Fax, 86-10-62565689;
e-mail, [email protected]